There have been angry clashes between Russian and US envoys at the UN Security Council, after the US called a meeting to discuss Moscow’s troop build-up on its borders with Ukraine.
US Ambassador Linda Thomas-Greenfield said the mobilisation was the biggest Europe had seen in decades.
Her Russian counterpart accused the US of fomenting hysteria and unacceptable interference in Russia’s affairs.
The US and UK have promised further sanctions if Russia invades Ukraine.
UK Foreign Secretary Liz Truss said legislation was being prepared which would target a wider range than currently of individuals and businesses close to the Kremlin.
A US official said Washington’s sanctions meant individuals close to the Kremlin would be cut off from the international financial system.
Russia has placed an estimated 100,000 troops, tanks, artillery and missiles near Ukraine’s frontiers.
At Monday’s UN Security Council meeting, Russian Ambassador Vasily Nebenzya said there was no proof that Russia was planning military action against Ukraine, and that its troop build-up was not confirmed by the UN.
He said Russia often deployed troops on its own territory and that this was none of Washington’s business.
Russia had tried to block the open session of the UN body but was outvoted by 10 votes to two.
The Biden administration was “whipping up tensions and rhetoric, and provoking escalation”, Mr Nebenzya said.
“This isn’t just unacceptable interference in the internal affairs of our state, it’s also an attempt to mislead the international community about the true situation in the region and the reasons for the current global tensions,” he said.
Ms Thomas-Greenfield said the US continued to believe there was a diplomatic solution but warned that the US would act decisively if Russia invaded Ukraine, the consequences of which would be “horrific”.
“This is the largest… mobilisation of troops in Europe in decades,” she said.
“And as we speak, Russia is sending even more forces and arms to join them.”
Moscow was planning to increase its force deployed in neighbouring Belarus, on Ukraine’s northern border, to 30,000, she added.
Late on Monday, the US ordered the departure of family members of American government employees from Belarus, citing the “unusual and concerning Russian military buildup”. A similar order was last month issued to families of US government personnel in the American embassy in Ukraine’s capital Kyiv.
Meanwhile diplomatic efforts continue, with Mr Putin speaking by phone to French President Emmanuel Macron on Monday.
US Secretary of State Antony Blinken will hold talks with Russian Foreign Minister Sergei Lavrov on Tuesday.
The US says it has received a written response from Russia to a US proposal aimed at de-escalating the crisis in Ukraine.
A state department spokesperson said it would be “unproductive” to negotiate the response in public. The spokesperson added that the US remained fully committed to dialogue and would continue to consult closely with its allies and partners, including Ukraine.
Moscow wants the West to promise Ukraine will never join the Nato alliance – in which members promise to come to another’s aid in the event of an armed attack – but the US has rejected that demand.
Nato’s 30 members include the US and UK, as well as Lithuania, Latvia and Estonia – former Soviet republics which border Russia. Moscow sees Nato troops in eastern Europe as a direct threat to its security.
Mr Putin has long argued the US broke a guarantee it made in 1990 that Nato would not expand further east, though interpretations differ over exactly what was promised.
Russia annexed Ukraine’s southern Crimea peninsula in 2014. It is also backing rebels who seized large swathes of the eastern Donbas region soon afterwards, and some 14,000 people have died in fighting there.